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. 2007 Oct 12;407(Pt 3):419–426. doi: 10.1042/BJ20070766

Figure 3. Purification of recombinant TcGAL.

Figure 3

Lanes 1–6, SDS/PAGE gel (10% polyacrylamide) stained with Coomassie Blue. Lane 1, size standards; lane 2, crude extract loaded on to a Ni-NTA column; lane 3, flowthrough. The column was washed extensively with 10 mM imidazole (lane 4, first wash; lane 5, last wash). Recombinant protein was eluted with 200 mM imidazole (lane 6). Lane 7, Western blot of samples from the 200 mM imidazole elution stage in TcGAL purification. Samples were Western-blotted and probed with Anti-Xpress® antibody to identify the recombinant protein. Sizes are indicated in kDa.