Table 2. FMN-binding properties of mutated TcGAL proteins.

Supernatants from TCA-precipitated TcGAL mutants (Figure 2) were analysed by HPLC. The resulting peaks had the same retention time as the FMN control (Figure 5). The extent of cofactor binding is expressed as a percentage of the peak height obtained with the same amount of wild-type (WT) enzyme (122 mg). Assays were performed in duplicate, and results are means±S.E.M.

Mutant	FMN peak height (%WT)
K55G	74.6±1.3
K55H	8.1±0.7
K55L	0
H447G	0
W448G	0
K450G	52.4±0.9