Figure 2.

Yields obtained from amplification of different amounts of input DNA. An input DNA sample was obtained by reverse crosslinking chromatin extracts from a wild-type yeast culture, and used as starting material for a double-round T7 amplification. (A) Averaged RNA yields after two rounds of amplification are plotted as function of the amount of starting material used, which ranged from 0.4 to 2.5 ng. The concentration of T7 oligo added to the Klenow reactions was scaled proportionally with the amount of starting material, and set to 25 nM and 250 nM for the first and second round, respectively. Assays were performed in duplicate and error bars indicate the standard deviation. (B) Analysis of fragment size of amplified material after single round or double round of T7 amplification. One hundred and fifty nanograms of each sample was analyzed on an Agilent 2100 Bioanalyzer together with a RNA size marker. The fragment sizes of the RNA marker are indicated.