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. 2008 Jan 7;36(4):e21. doi: 10.1093/nar/gkm1144

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Double-round T7 amplification results in reduced technical variation compared to LM-PCR. Histograms of averaged binding ratios of four hybridizations with double-T7 (A) or LM-PCR amplified samples (B) across all DNA microarray probes. (C) Overview plot of chromosome 7 showing locations of array probes (black) and genes (green). Graphs of binding ratios of input versus ChIP show the background and binding signals for double-round T7 amplification (double-T7) (top) and LM-PCR (bottom). (D) Enlargement of indicated regions in (C).

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