Figure 5.

Quantitative PCR validation of Gsm1p-bound regions identified after double-round T7 amplification and LM-PCR amplification. Quantitative PCR validation of Gsm1p-binding peaks identified by both double-round T7 amplification (double-T7) and LM-PCR (A), LM alone (B) or double-T7 alone (C). Binding to tested regions is represented as enrichment over the HMR silent mating-type locus. (D) Enlarged overview of Gsm1p-binding ratios on the indicated loci using either double-T7 (red) or LM-PCR (blue). Locations of array probes (black) and genes (green) are indicated.