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. 2008 Jan 7;36(4):e21. doi: 10.1093/nar/gkm1144

Table 1.

Quantification of single and double round T7 amplification of input ChIP DNA

Input (ng) Yield after single T7 (ng) Yield after double T7 (ng)
0 <100a 112
0.4 <100a 4960
1 <100a 20160
2.5 <100a 35840
5 420 50240b
20 2480 NDc
80 9880 NDc

aexact quantification was not possible because of low yield.

b150 ng from single T7 was used for second round of double T7.

cNot determined.

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