Table 1.
Quantification of single and double round T7 amplification of input ChIP DNA
Input (ng) | Yield after single T7 (ng) | Yield after double T7 (ng) |
---|---|---|
0 | <100a | 112 |
0.4 | <100a | 4960 |
1 | <100a | 20160 |
2.5 | <100a | 35840 |
5 | 420 | 50240b |
20 | 2480 | NDc |
80 | 9880 | NDc |
aexact quantification was not possible because of low yield.
b150 ng from single T7 was used for second round of double T7.
cNot determined.