Table 2.

Quantification of single and double round T7 amplification of several ChIP samples

ChIP sample	Culture volume (ml) (OD600 = 0.6)	Yield after ChIP (ng)	Yield after single T7 (ng)	Yield after double T7 (ng)
H3	200	140	18 000	NDa
pol II	200	<20b	500	31 680c
Prot A	200	<20b	144	32 000
TBP	400	<20b	784	46 080c
TBP	200	<20b	300	21 840c
TBP	100	<20b	200	22 880c
TBP	25	<20b	150	37 600

^aNot determined.

^bExact quantification was not possible because of low yield.

^c150 ng from single T7 was used for second round of double T7.