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. 2007 Dec 23;36(4):1153–1162. doi: 10.1093/nar/gkm1113

Figure 3.

Figure 3.

Expression of four miR-122 targets. (A) Alignment showing the seed matches in Cd320, Ndrg3, AldoA and Bckdk. The 6-nt seed is shown in capital letters, with the m8M or t1A extensions highlighted in red. (B) Real-time RT-PCR analysis of AldoA, Nrdg3, Bckdk and Cd320 was carried out to investigate their expression as response to increasing LNA-antimiR doses. Shown are mean and SD (where n = 3) of mRNA levels relative to the saline control group. Shown is also the mismatch control (mm). (C) miR-122-mediated repression of 3′UTR reporters for AldoA, Ndrg3, Bckdk and Cd320 in HeLa cells. The 3′UTRs of the four predicted miR-122 targets were cloned downstream of the Renilla luciferase gene and the resulting reporters were transfected into HeLa cells along with a miR-122 mimic. Shown are mean values and SD, n = 4. (D) Western blot analysis of AldoA in liver protein extracts of LNA-antimiR-treated and saline control mice. Shown is one representative western blot and corresponding quantification. Values are related to the mean of the saline values (mean and SD, n = 4, asterisk: 75 µg protein instead of 100 µg, compensated for in quantification).