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. 2008 Jan 3;36(4):1273–1287. doi: 10.1093/nar/gkm1140

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Isothermal titration calorimetric analysis for the binding of hLon to LSPas and TG₆T. (A) LSPas and (D) TG₆T were titrated (350 µM) into solutions containing hLon (30 µM); heat responses were registered isothermally at the temperatures as indicated. Integrals of the heat responses were normalized and fitted with a nonlinear least-square method to obtain thermodynamic parameters (B and E). The parameters including −TΔS, ΔH and ΔG representing the association of hLon with LSPas (C) and TG₆T (F) were plotted against temperature.