Table 2.

Thermodynamic parameters for the binding of hLon to LSPas and TG₆T

Target DNA	Temperature (°C)	ΔH (kcal/mol)	−TΔS (kcal/mol)	Ka (×105 M−1)	ΔG (kcal/mol)	ΔCp (cal/mol/K)
LSPas	10	−1.48 ± 0.12	−5.49	2.45 ± 0.43	−6.99 ± 0.09	−607.82
	15	−5.04 ± 0.12	−1.98	2.11 ± 0.11	−7.03 ± 0.03
	20	−8.37 ± 3.31	1.01	3.03 ± 0.83	−7.29 ± 0.17
	30	−13.72 ± 6.91	6.21	2.60 ± 0.72	−7.52 ± 0.15
TG6T	5	−2.04 ± 0.75	−4.14	1.24 ± 0.58	−6.49 ± 0.21	67.29
	10	−1.84 ± 0.88	−4.71	1.35 ± 0.89	−6.58 ± 0.36
	20	−1.30 ± 0.14	−5.69	1.36 ± 0.22	−6.80 ± 0.09
	30	−0.63 ± 0.02	−7.05	3.48 ± 0.36	−7.69 ± 0.06

ΔC_p was obtained from linear regression of ΔH against temperature. The binding thermodynamic parameters of both LSPas and TG₆T were calculated on the monomeric concentrations of hLon and DNA. The LSPas constants corrected for the G-quartet fraction of LSPas are aΔH, −T [ΔS + (a/T + Rlna)], alnK, ΔG-RTlna and aΔC_p, respectively. a represents the reciprocal of the LSPas G-quartet fraction, which was estimated 1.15 as analyzed by ImageQuant ver. 5.2 (Molecular Dynamics) with Figure 3A lane 1 (G-quartet 0.87; monomer 0.13).