Representative tracings of the Isc response of
rat jejunum to the serosal addition of (A)
SL-NH2 (20 μM) and LS-NH2 (80 μM), when
tissues were bathed in normal Krebs buffer, or SL-NH2 (80 μM) in
tissues bathed with chloride- (Cl−) free Krebs buffer.
(B) tc-NH2 (40 μM) and trypsin (10 and 50
units/ml; 20 and 100 nM) in normal Krebs buffer. (C)
Cit-NH2 (20 and 100 μM) and thrombin (5 units/ml; 50
nM) in normal Krebs buffer. (D) SL-NH2 (80 μM)
after trypsin (100 units/ml; 200 nM), and trypsin alone (100
units/ml; 200 nM), followed by carbachol (Cch, 1 μM) in normal
Krebs buffer. The scale for time and Δ Isc (in
μA/cm2) is shown respectively to the right and to the
left of each tracing. All panels show a tracing for an individual
tissue preparation that is representative of six independently
conducted experiments.