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. 2008 Feb 5;36(4):e23. doi: 10.1093/nar/gkn018

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — The predicted circadian enhancer region is essential for robust circadian oscillation driven by the mDbp promoter. (A) Deletions of the mDbp promoter: Dbp(−462):Luc contains the whole predicted enhancer region, Dbp(−410):Luc contains only a part of the predicted enhancer region. (B) Transcriptional assay using these reporter constructs. Both Dbp:Luc and Dbp(−462):Luc are strongly up-regulated (16-fold and 19-fold induction, respectively) by co-expression of the CLOCK/BMAL1 proteins, whereas the Dbp(−410):Luc reporter is induced only about 2-fold induction in response to CLOCK/BMAL1 co-expression. (C) The Dbp(−410):Luc-driven bioluminescence rhythm exhibits a dampened oscillation of very low amplitude; in contrast, both Dbp:Luc and Dbp(−462):Luc drive robust circadian oscillation of bioluminescence.