Figure 5.

In vivo functional analysis of the RRM3 mutants. (A) Schematic representation of the UnGA reporter gene. The UnGA has the alternatively spliced intron from Nrg whose splicing is regulated by ELAV. It is transcribed ubiquitously, but GFP is expressed only when ELAV promotes the neural splicing of the intron. (B) Ectopic expression of ELAV in the wing disc by the dpp-GAL4 driver leads to the GFP expression from UnGA. Anti-ELAV (left panel) and anti-GFP (right panel) staining of a UnGA/+; dpp-GAL4/UAS-eQ12H3 wing disc are shown. The outline of the disc is shown with a white line. (C) The wild-type control and three mutant forms of ELAV were expressed by the dpp-GAL4 driver. Two independent insertion lines were used for each UAS construct. GFP expression levels in the wing disc were quantified, and normalized by ELAV expression levels. The bars on the right panel show the averaged values of eight wing discs for each genotype, as fractions of the mean value of the wild-type controls. The error bars show the 95% confidence intervals. Bars labeled with different letters (a–e) are significantly different at P < 0.01 in the t-test.