Table 1.

The design of mouse BMP-4 and random molecular beacons and their computed melting temperature

Beacon ID	Molecular Beacon Design	Melting temperature (°C)
		MB	Duplex
MB1	5′-Cy3-CCCGTTATAATAACAGTCCATACGGG-BHQ2-3′	56.0	45.4
MB2	5′-Cy3-CGGACTCTGTAGAAGTGTCGCCGTCCG-BHQ2-3′	52.4	59.4
MB3	5′-Cy3-CCCACGGAGCCGGTAAAGATGTGGG-BHQ2-3′	57.6	55.8
MB4	5′-CY3-CCAGCCTGCTCTTCCTCCTCCTCGCTGG-BHQ2-3′	52.3	62.4
MB5	5′-Cy3-CGCTCCCCGGTTCCCTGGCTCGAGCG-BHQ2-3′	54.8	66.3
MB6	5′-CY3-CACCCTAACGATCGGCTGATGGGTG-BHQ2-3′	55.3	54.5
MB7	5′-Cy3-CGCTCCCCAATCTCCACTCCGAGCG-BHQ2-3′	55.7	56.6
MB8	5′-Cy3-CGCAGCCTCTACCACCATCTCCCTGCG-BHQ2-3′	55.4	57.5
MB8a	5′-Cy3-CGAGCCCATCTCCTGATAATTTGCTCG-BHQ2-3′	55.2	51.4
MB8b	5′-Cy3-CCGACCACCCCTCTACCACGTCGG-BHQ2-3′	55.0	55.3
Random MB	5′-Cy3-CGACGCGACAAGCGCACCGATACGTCG-BHQ2-3′	57.7	NA