Table 3.
Predicted RNA-binding residues in aminoacyl–tRNA–synthetases
PDB-chain | Binding site | Predicted RNA-binding residues |
---|---|---|
1h3f-A | Patch | I168−Y175, A178, Q179 |
Clefta | L42, G43–D45, P46−D50, H52, G54, H55, V58, G77, F79, Y108, Q111, R155−D157, H171, E172, Y175, A178, Q179, G194, D196, Q197, N200, P222, L223, V225, R230, E231, K232, S234, K235, S236, I237, Y240, T244 −P246 | |
1hc7-A | Patchb | S15, L19, Y30–T36, S88, E90, L91, E113, T114, R142, W143, E144, M145–R148, L151, R152, E155, F156, L157, W158, K199, K202–F205, A206, G207, Q225, A226, T228, H230, L232, N235, F236, S258, G260, S262, W263, R264, Q437, E438, T441, T443, A476, Y477 |
Cleft | I37–V39, Y44, L70, F71, F87, P89, A92, V93, V108, N139, V141, W143, E155, L157 | |
1j09-A | Patch | L235, R237, N238, P239, D240, K241, T242, K243, I244, S245, K246, R247, K248, S249, H250 |
Cleftc | A7, S9, P10, T11, G12, D13, H15, G17, T18, I21, E41, D42, T43, D44, R45, A46, R47, V49, K180, Y184, T186, Y187, A206, E208, W209, L235, K243, I244, S245, K246, R247, S249, H250, S252, W255 |
aResidues in bold underlined are involved in binding ATP or tyrosinol based on the corresponding structure of tyrosyl-tRNA synthetase complexed with its cognate tRNATyr, ATP and tyrosinol (1h3e-A).
bResidues in bold underlined are involved in binding ATP or prolinol based on the corresponding structure of prolyl-tRNA synthetase complexed with its cognate tRNAPro, ATP and prolinol (1h4q-A).
cResidues in bold underlined are involved in binding the tRNA 3′-terminal CytCytAde based on the structure of glutamyl-tRNA synthetase complexed with its cognate tRNAGlu and glutamol-AMP (1n78-A).