Skip to main content
. 2008 Jan 18;36(5):1482–1496. doi: 10.1093/nar/gkm1178

Figure 4.

Figure 4.

Evaluation of inhibition kinetic parameters of coumestan compounds with regard to UTP substrate. Reaction velocities were measured at increasing concentrations of UTP substrate (2.5–80 μM) on poly rA-U12 template/primer (0.25 μM) in the absence or presence of increasing concentrations (5–140 μM) of the indicated inhibitor. Panels A, B and C depict the Dixon and Cornish-Bowden plots for wedelolactone, LQB34 and LQB96, respectively. Both plots display similar intercept profiles on the x-axis indicating a noncompetitive mode of inhibition with regard to UTP. The Ki values corresponded to 8.4, 4.2 and 42 μM for wedelolactone, LQB34 and LQB96, respectively.