Skip to main content
. 2008 Jan 21;36(5):1555–1566. doi: 10.1093/nar/gkm1173

Figure 3.

Figure 3.

Inhibition of APE1 promoter activity by WT but not mutant p53. (A) p53(−/−) cells were transiently transfected with (0.5 μg) of APE1 promoter–luciferase plasmid (−4500/+65) and various amounts of WTp53 expression plasmid or control vector. Luciferase activity was measured at 48 h after transfection and normalized for the amount of protein. The mean ± SD of five independent experiments performed in duplicate is shown. (B) Western analysis of the p53 level in the transfected cell extracts used in (A). (C) p53(−/−) cells were cotransfected with (0.5 μg) of p21 promoter–luciferase plasmid and WTp53. Luciferase activity was measured 48 h after transfection and normalized for the amount of protein. (D) p53(−/−) cells were transiently transfected with (0.5 μg) of APE1 promoter–luciferase plasmid (−4500/+65) and 0.05 μg of expression plasmid encoding WTp53 or mutant p53 (V143A) (22,23) or equivalent amount of empty vector. Other details are given above. (E) Western analysis of WT and mutant p53 in cells lysates used in (D); *P < 0.05; **P < 0.001.