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. 2008 Jan 21;36(5):1555–1566. doi: 10.1093/nar/gkm1173

Figure 6.

Figure 6.

ChIP assay for in vivo association of p53 with APE1 promoter. (A) EMSA of purified p53 (60 ng, lane 4; 120 ng, lane 5) using 32P-labeled duplex oligo corresponding to the bases (−184 to 142) from the APE1 promoter sequence or containing consensus p53-binding sequence from the p21 promoter (lane 2) as a control. (B) p53(+/+) cells were treated with CPT for 9 h, the protein–DNA was crosslinked, and ChIP assays performed as described in Materials and Methods section. Immunoprecipitated APE1 promoter with the indicated antibody was amplified with primers as described in Materials and Methods section. Left panel, schematic diagram of the APE1 promoter showing the relative position of PCR primers used in the ChIP assays.