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. 2008 Jan 21;36(5):1555–1566. doi: 10.1093/nar/gkm1173

Figure 7.

Figure 7.

Effect of TSA on p53-mediated repression of APE1 promoter activity. (A) p53(−/−) cells were transiently transfected with 0.5 μg of APE1 promoter–luciferase plasmid (−1800/+65) and increasing amounts of expression plasmid encoding WTp53 or equivalent amounts of empty vector. Twenty-four hours after transfection cells were treated with or without TSA (100 ng/ml) and luciferase activity was measured 48 h after transfection and normalized for the amount of protein. The mean ± SD of five independent experiments performed in duplicate is shown. (B) Sequence of the DNA probes for EMSA assay with APE1 promoter. Schematic diagram of the APE1 promoter showing some of the putative transcription factor binding sites (Sp1; USF; glucocorticoid response element (GRE); activator protein 1 (AP-1); and CCAAT box-like sequence). (C) EMSA with nuclear extracts of p53(−/−) cells transfected with empty vector or WTp53 expression plasmid using 32P-labeled duplex oligo corresponding to bases −177 to −143 from APE1 promoter; *P < 0.05.