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. 2008 Jan 18;36(5):1464–1471. doi: 10.1093/nar/gkm1154

Figure 4.

Figure 4.

Strand invasion in G–C-rich regions by pcPNA strands involving chiral PNA monomers; (a) invasion site = (G/C)7(A/T)3 and (b) (G/C)8(A/T)2. Lane 1, DNA only (control); lane 2, conventional pseudo-complementary D–Us's on N-(2-aminoethyl)glycine; lane 3, chiral D–Us's on N-(2-aminoethyl)-d-lysine. Invasion conditions: (i) [DNA1] = 5 nM and [PNA (each strand)] = 50 nM at pH 7.0 (HEPES buffer) and 50°C for 1.5 h; (ii) [DNA1] = 5 nM and [PNA (each strand)] = 30 nM. The gel-shift assay was performed at 20°C.