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. 2008 Jan 18;36(5):1464–1471. doi: 10.1093/nar/gkm1154

Figure 5.

Figure 5.

Promotion of invasion to (G/C)3(A/T)7 site by introducing chiral PNA monomers to the pcPNA strands. In lane 3, two conventional A–T pairs (in blue) of the PNA in lane 2 are replaced with chiral A–T pairs (in red). In lane 4, simple lysine residues (Figure 3b, II) were bound to the termini of the pcPNAs used in lane 2 so that the net positive charges (+10) were the same as those in lane 3. Invasion conditions: [DNA2] = 10 nM and [PNA (each strand)] = 200 nM at pH 7.0 (HEPES buffer) and 50°C for 1.5 h. The gel-shift assay was performed at 20°C.