Figure 2.

Expression of the ahdI genes in vivo. (A) The horizontal lines show overnight 37°C growth of E. coli Z85 strain harbouring indicated plasmids on an LB agar plate. Cells were spotted with indicated dilutions of λ-vir phage lysate. (B and C) Primer extension analysis of ahdI transcripts. RNA was purified from E. coli Z85 strain harbouring wild-type AhdI plasmid pAhdIRM (lanes 1) or pAhdIRM derivative with disrupted ahdIC (lanes 2) and primer extension reactions were performed with oligonucleotide primers complementary to the ahdIC (Figure 2B) and ahdIM (Figure 2C) genes. Sequencing reactions marker lanes were prepared with pAhdIRM and primers used for primer extension.