Table 3.

Mismatch extension ability of the wild-type and K1061A mutant pol ζ proteins

Primer- terminal base paira	Wild-type		K1061A		Decreasec
	kcat/Km (μM/min)	f °extb	kcat/Km (μM/min)	f °extb
G·G	0.26	0.11	8.6 × 10−3	0.062	1.8
A·G	1.0	0.43	7.4 × 10−3	0.053	8.1
T·G	0.23	0.10	2.5 × 10−3	0.018	5.6
C·G	2.3	NA	0.14	NA	NA
G·A	0.36	0.33	3.8 × 10−3	0.019	17
A·A	0.44	0.40	7.8 × 10−3	0.037	11
T·A	1.1	NA	0.21	NA	NA
C·A	0.40	0.36	1.9 × 10−3	9.0 × 10−3	40
G·T	0.56	0.081	9.3 × 10−3	0.026	3.1
A·T	6.9	NA	0.36	NA	NA
T·T	0.13	0.019	2.4 × 10−3	6.7 × 10−3	2.8
C·T	0.23	0.033	4.1 × 10−3	0.011	3.0
G·C	4.3	NA	0.19	NA	NA
A·C	2.1	0.49	0.018	0.095	5.2
T·C	1.0	0.23	6.9 × 10−3	0.036	6.4
C·C	0.20	0.047	6.7 × 10−4	3.5 × 10−3	13

NA, not applicable.

^aThe primer-terminal base pairs are given as primer strand residue followed by template strand residue.

^bThe relative efficiency (f°_ext) for extending from a given primer-terminal mismatch is equal to the k_cat/K_m for extending from the mismatched primer-terminal base pair divided by the k_cat/K_m for extending from the corresponding matched primer terminal base pair.

^cThe fold decrease in mismatch extension ability of the K1061A mutant protein relative to the wild-type protein is equal to the f°_ext for the wild-type protein divided by the f°_ext for the mutant protein.