Table 2.

Mature human let-7 family sequences in DNA and their hybridization T_m with perfectly complementary pairs

Name (control)	Sequencea	Tm (°C)b
		let-7a	let-7b	let-7c	let-7d	let-7e	let-7f	let-7g	let-7i	miR-98
let-7a	TGAGGTAGTAGGTTGTATAGTT	64	58	59	58	57	59	52	51	51
let-7b	TGAGGTAGTAGGTTGTG TG GTT	58	70	65	51			51	54
let-7c	TGAGGTAGTAGGTTGTATGGTT	59	64	67	51	51	52	52	51	51
let-7d	AGAGGTAGTAGGTTGCATAGT	55	51	51	66
let-7e	TGAGGTAGGAGGTTGTATAGT	62	55	57	56	66	55
let-7f	TGAGGTAGTAGATTGTATAGTT	57					62
let-7g	TGAGGTAGTAGTTTGTACAGT							64	55
let-7i	TGAGGTAGTAGTTTGTGCTGT							55	68
miR-98	TGAGGTAGTAAGTTGTATTGTT									63

^aMismatch sequences compared to let-7a are shown in bold italics.

^bHybridization T_m to the target is in bold; 51°C in italics, for reference, is not expected to produce signals in our design criteria.