Figure 1.

NF-YB RNAi in HCT116 cells. (A) Three siRNA were designed on human NF-YB gene. Black boxes represent exons; red boxes indicate targeted exons. (B) Left panel: RT-PCR analysis of NF-YB and GAPDH mRNA transcripts in negative control and NF-YB1-2-3-silenced cells. Right panel: western blot analysis of HCT116 total extracts with anti-NF-YB and anti-actin antibodies, transfected with control and NF-YB siRNAs. (C) RT-PCR (left panel) and western blot analysis (right panel) of the three NF-Y subunits in control or NF-YB2 transfected cells. (D) Chromatin immunoprecipitation analysis of NF-Y targets, using control and NF-YB-silenced HCT116 cells with NF-YB and Flag antibodies.