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. 2008 Jan 10;36(5):1415–1428. doi: 10.1093/nar/gkm1046

Figure 5.

Figure 5.

NF-YB silencing induces apoptosis via caspases activation. (A) Cell cycle distribution analysis via flow cytometry of non-targeting control and NF-YB siRNA transfected cells, untreated or treated with ZVAD. (B) Western blot analysis of negative control and NF-YB-silenced HCT116 total extracts with anti-NF-YB and anti-actin antibodies, untreated or treated with ZVAD. (C) Expression analysis (RT-PCR) of the indicated caspase mRNA transcripts. (D) Immunofluorescence analysis of the indicated cleaved caspases and HOECHST.