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. 2008 Jan 10;36(5):1415–1428. doi: 10.1093/nar/gkm1046

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Role of p53 in apoptosis induction. (A) Expression levels of NF-YB and actin in HCT116/E6 upon NF-YB silencing. (B) Cell cycle progression analysis by FACS of control and NF-YB-silenced HCT116/E6 cells. Error bars indicate standard deviations. (C) Left panel: RT-PCR analysis of G2/M, pro- and anti-apoptotic genes upon NF-YB siRNA transfection. PCR amplified genes are indicated and their quantitation, relative to control expression levels, is plotted in the right lower panel. Right upper panel: total extracts subjected to western immunoblotting using anti-bax and anti-actin antibodies. (D) Dose–response analysis (50–100 ng) of NF-Y in HCT116/E6 cells with Bcl-2 reporter. Error bars indicate standard deviations (E) The histogram represents the Bax/Bcl-2 and Bax/Bcl-X_L mRNA ratios in HCT116 and HCT116/E6 NF-YB-silenced cells.