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. 2008 Feb 7;36(5):1690–1702. doi: 10.1093/nar/gkn009

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Full-length Glis3 functions as a potent transcriptional activator. HEK293T cells were co-transfected with p3×FLAG-CMV-Glis3, p3×FLAG-CMV-Glis3(ΔN155), p3×FLAG-CMV-Glis3(ΔN463), p3×FLAG-CMV-Glis3(ΔC756), pCMVβ and (Glis-BS)₆-LUC as indicated. After 30 h, cells were assayed for luciferase (LUC) and β-galactosidase activity. The relative LUC reporter activity was calculated and plotted. A schematic view of the deletion mutants is shown below the graph. AD, activation domain; ZDF, zinc finger domain.