Skip to main content
. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Free Radic Biol Med. 2007 Sep 25;44(5):768–778. doi: 10.1016/j.freeradbiomed.2007.09.011

Figure 6. Inhibitors of mitochondrial GSH transport potentiate MQ-induced increases in cytosolic and mitochondrial GSSG and protein-SSG: NAC attenuates cytosolic, but not mitochondrial changes.

Figure 6

NCM460 cells were pre-treated with or without 20mM BM/PS for 1h and then incubated for 30min with 50µM MQ. Wherever present, 2mM NAC was added at 30min prior to MQ exposure. Cytosolic and mitochondrial fractions were obtained by digitonin fractionation and GSH and GSSG contents were determined in each compartment. Mitochondrial protein-SSG were also determined. Results are expressed as mean ± S.E. for n=3 for control and MQ without or with BM/PS, or n=4 for BM/PS + NAC. A, GSH, *p< 0.05 versus control; #p< 0.05 versus BM/PS; op< 0.05 versus BM/PS+MQ. B, GSSG, *p< 0.05 versus control; #p< 0.05 versus +MQ. C, mitochondrial protein-SSG contents. *p< 0.05 versus control.