Table 4.
A comparison between the different heterokaryon incompatibility and programmed cell death related genes in the two sequenced A. niger genomes (CBS513.88 and ATCC1015). If two proteins differ in size, the longer one is the basis for counting percentage of identities, similarities and gaps. Gaps are counted only in the homologous region.
Function | Protein | Type and size of difference | Identities | Gaps | ||
---|---|---|---|---|---|---|
Heterokaryon incompatibility genes (N. crassa & P. anserina) | ||||||
HET-6 | only HET domain motifs are slightly conserved | - | - | |||
HET-C | indel: 622 | 791/793 (99 %) | 1/793 | |||
substitution: 196 | (0 %) | |||||
UN-24 | no difference | (100 %) | (0 %) | |||
HET-C2 | no difference | (100 %) | ||||
HET-D / HET-E | diverse proteins with WD40 repeats, but no remarkable similarity | - | ||||
Suppressor genes (N. crassa) | ||||||
TOL | very diverse proteins, with conserved HET domain motifs | - | - | |||
VIB-1 | substitution: 195 | 585/586 (99 %) | (0 %) | |||
Incompatibility related genes (N. crassa & P. anserina) | ||||||
HAM-2 | substitution: 756 | 1066/1067 (99 %) | (0 %) | |||
PIN-C | very diverse proteins, with conserved HET domain motifs | - | - | |||
RNR-A | no difference | (100 %) | (0 %) | |||
IDI-6 / PSP | 1st pair: indel: 534 | 531/535 (99 %) | ||||
(2 alleles) | substitutions: 398, 525 | |||||
2nd pair: substitutions: 398, 436-443, 450-459, 471-514 | 413/416 (99 %) | |||||
IDI-7 | no difference | (100 %) | ||||
Modifier genes (P. anserina) | ||||||
MOD-D (2 alleles) | Members of the two allele pairs are 100 % identical, between the pairs there are some difference: 1-167 variable part, 1-60 and 168-360 more conserved region. | |||||
MOD-E | substitution: 244 | 672/702 (96 %) | (0 %) | |||
Programmed Cell Death genes (S. cerevisiae) | ||||||
ATP4 | no difference | 100 % | 0 % | |||
CDC48 | no difference | 100 % | 0 % | |||
HEL 13 | present only in CBS 513.88 | - | - | |||
MCA1 / YCA1 | 1st pair 438/438 (100 %) | (0 %) | ||||
(2 alleles) | ||||||
2nd allele: indel region: 1-56, 75-104, 237, 443-447 | ||||||
subtitutions: 57-75, 105-106. 236, 440-442 | 2nd pair 341/441 (77 %) | 1/333 (0 %) | ||||
NSR1 | diverse proteins, with short conserved motifs | - | - | |||
PPA1 | no difference | 100 % | 0 % | |||
SAR1 | no difference | 100 % | 0 % | |||
STM1 | indel region: 8-13 | 297/303 (98 %) | 6/303 | |||
(1 %) | ||||||
TOR1 | substitution: 16 | 2389/2390 (99 %) | (0 %) |