Figure 3.

The decrease in the surface expression of Flag-GluR1 clusters at synapses is activity- and time-dependent. The Na⁺ channel toxin tetrodotoxin (A) or the NMDAR antagonist d-APV (B) largely prevented the picrotoxin-induced decrease in the proportion of synapses containing surface clusters of Flag-GluR1. Each graph illustrates the percentage of synaptophysin puncta that colocalize with Flag-GluR1. (C) The time course of the picrotoxin-induced effect. The abcissa shows the duration of picrotoxin treatment. Error bars represent SEM.