Table 3.

Antiendomysial (EmA) and transglutaminase-2 antibody (TG2-ab) secretion to organ culture supernatants after in vitro PT-gliadin challenge. Organ culture of treated celiac disease patient biopsy samples (n = 9) who had no small-bowel mucosal IgA deposits. Small-intestinal biopsy samples were cultured either with medium only or with PT-gliadin for 24 or 48 hours. EmA titers were graded according to intensity of staining as follows: negative (neg), weak positive (+) and strong positive (++, +++ or ++++).

		EmA				TG2-ab (U)
Patient	GFD years	Medium 24 h	PT-gliadin 24 h	PT-gliadin 48 h		Medium 24 h	PT-gliadin 24 h	PT-gliadin 48 h
12	1	neg	neg	neg		2.0	4.8	13.3
13	2	neg	neg	neg		8.8	6.9	4.4
14	4	neg	neg	neg		2.2	6.2	10.3
15	4	neg	neg	neg		2.5	1.7	2.6
16	6	neg	neg	neg		8.1	7.7	12.4
17	7	neg	neg	neg		2.4	3.7	1.9
18	8	neg	neg	neg		2.5	1.3	1.7
19	14	neg	neg	neg		0.9	2.9	0.7
20	20	neg	neg	neg		11.6	2.5	11.6
Median		neg	neg	neg	Median	2.5	3.7	4.4*

GFD = gluten-free diet

* P > 0.05 compared to medium only 24 hours and PT-gliadin 24 hours