Table 4.
Analysis of molecular variance (AMOVA) for msh1 and 18S (in parenthesis).
| Comparison tested | d.f. | Sum of squares | Variance component | % variation | ΦST |
|---|---|---|---|---|---|
| Between habitats (shallow Vs deep) | |||||
| Among populations | 1 (1) | 84. 89 (55.599) | 1.29890 (0.79038)Va | 66.87 (49.27) | 0.66873 (0.49266)*** |
| Within populations | 128 (141) | 82.36 (114.765) | 0.64345 (0.81394)Vb | 33.13 (50.73) | |
| Total | 129 (142) | 167.254 (170.364) | 1.94235 (1.60431) | ||
| Between morphotypes (shallowtype Vs deeptype) | |||||
| Among populations | 1 (1) | 130.412 (108.532) | 2.06222 (1.59683)Va | 87.89 (78.45) | 0.87891 (0.78455)*** |
| Within populations | 128 (141) | 36.084 (61.832) | 0.28413 (0.43852)Vb | 12.11 (21.55) | |
| Total | 129 (142) | 167.254 (170.364) | 1.94235 (2.03536) | ||
| Between reefs (Romero Vs Media Luna) | |||||
| Among populations | 1 (1) | 1.702 (0.642) | 0.00829 (0.01242)Va | 0.64 (0.96) | 0.00637 (0.01043) |
| Within populations | 128 (107) | 165.552 (128.744) | 1.29338 (1.20321)Vb | 99.36 (99.04) | |
| Total | 129 (108) | 167.254 (129.385) | 1.94235 (1.19079) | ||
| Between reefs considering shallow populations only (Romero Vs Media Luna Vs Culebra) | |||||
| Among populations | 2 (2) | 0.921 (1.790) | 0.00157 (0.00257)Va | 0.37 (0.30) | 0.00366 (0.00305) |
| Within populations | 65 (74) | 27.844 (62.210) | 0.42837 (0.84067)Vb | 99.63 (99.70) | |
| Total | 67 (76) | 167.254 (64.000) | 1.94235 (0.84324) | ||
| Among reefs with different depth profiles (Media Luna Vs Romero Vs Culebra) | |||||
| Among populations | 2 (2) | 15.034 (4.639) | 0.18265 (0.03526)Va | 13.22 (2.91) | 0.13223 (0.02911)*** |
| Within populations | 127 (120) | 152.22 (141.101) | 1.19859 (1.17584)Vb | 86.78 (97.09) | |
| Total | 129 (122) | 167.254 (145.740) | 1.94235 (1.21110) | ||
Comparisons of population were made among depth profiles, morphotypes and reefs. The significance of the ΦST values were obtained by randomizations of 10,000 permutations. P < 0.001 is represented by ***. Sampling in Culebra is limited to shallow habitats.