Table 3.
CD4+ clones recognized colon cancer lines but not normal B or fibroblast cells sharing MHC class II molecules
| T cellb |
|||||||
|---|---|---|---|---|---|---|---|
| TIL
|
PBL
|
||||||
| Stimulator | Antibodya | HLA-DRβ1 genotype | C5 | C15 | C4 | C49 | C111 |
| None | None | <8c | <8 | <8 | <8 | <8 | |
| 1869 col CIITA | None | *0402, *1301 | 8695 | 1259 | 12328 | 12749 | 15269 |
| L243c | 279 | 162 | 511 | 524 | 790 | ||
| 1847 col + IFN-γd | None | *0401, *1301 | 2008 | 457 | 598 | 9758 | 11576 |
| L243 | 2055 | 327 | 585 | 790 | 2938 | ||
| 1872 col + IFN-γ | None | *0401, *1501 | 72 | <8 | 61 | <8 | 66 |
| L243 | 75 | <8 | 60 | <8 | 41 | ||
| 1869 EBV-B | None | *0402, *1301 | 79 | 116 | 122 | 232 | 209 |
| 1519 EBV-B | None | *0701, *1301 | 112 | 24 | 99 | 106 | 220 |
| 1519 Fibroblast + IFN-γ | None | *0701, *1301 | <8 | <8 | <8 | 55 | 62 |
| 1869 CD40LBe | None | *0402, *1301 | <8 | <8 | <8 | <8 | 45 |
| Stimulator | Antibodya | T cellb | |||||
|
|
|||||||
| C111 | |||||||
| None | 23c | ||||||
| 1869 col CIITA | 15269 | ||||||
| 1869 col CIITA | L243 | 790 | |||||
| 1681 mel + IFN-γd | 10298 | ||||||
| 1681 mel + IFN-γ | L243 | 253 | |||||
| 1869 CD40L-Be | 65 | ||||||
| 1681 CD40L-Be | 22 | ||||||
a
Where indicated, target cells were preincubated for 1 h with the anti-HLA DR mAb L243.
b
2 × 104 of the indicated T cells were incubated with 5 × 104 target cells in flat bottomed 96-well plate in 250 μl of AIMV 2% HS. After 18 h the supernatants IFN-γ secretion was evaluated by ELISA.
c
pg/ml of IFN-γ.
d
Where indicated, target cells were preincubated for 48 h with 500 IU of IFN-γ.
e
B cells from patients 1869 and 1681 were in vitro cultured with CD40L (100 IU/ml) and IL-4 (100 IU/ml).