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. Author manuscript; available in PMC: 2008 Mar 26.
Published in final edited form as: J Immunother. 2004;27(2):79–91. doi: 10.1097/00002371-200403000-00001

FIGURE 3.

FIGURE 3

Recognition of truncated gp100:420–440 peptides. PBMCs from patient 3 were stimulated three times with recombinant human gp100 protein and were subsequently cloned by limiting dilution. INF-γ secretion (pg/mL) by one CD4+ T cell clone was measured in response to various concentrations of the parent 20-mer as well as two truncated forms of the peptide, gp100:420–437 and gp100:420–435. INF-γ secretion (pg/mL) was also measured in response to 50 μmol/L Ig kappa chain:188–201 as a negative control.