TABLE 2.

Recognition of Known HLA-DRβ1^*0401 and HLA-DRβ1^*0701 Restricted gp100 Peptides, Transfectants, and CIITA Transduced Melanoma Cells by T Lymphocytes From Patients Expressing Both of These MHC Molecules Stimulated With Recombinant gp100 Protein^*

Target Cells	HLA-DR Expression	Peptide or Protein†	Introduced Gene†	Patient 1 (66% CD4+)	Patient 1 (77% CD4+)	Patient 2 (86% CD4+)	Patient 3 (100% CD4+)	Patient 4 (97% CD4+)	Targets Only
Media	None	—	—	44	0	0	14	0	0
293-DR4	DR4+	—	MART-1	32	4	12	16	10	26
293-DR4	DR4+	—	gp100	2,986	0	21	11	68	10
293-DR7	DR7+	—	MART-1	33	0	15	5	12	0
293-DR7	DR7+	—	gp100	36	10,041	1,138	1,674	7	16
1088 EBV-B	DR4+	HA:306–324	—	73	22	24	145	6	15
1088 EBV-B	DR4+	gp100:44–59	—	864	23	26	124	1,546	24
1978 EBV-B	DR7+	IgK:188–201	—	81	21	29	27	23	32
1978 EBV-B	DR7+	gp100:170–190	—	65	8,062	306	35	14	21
F002Rmel CIITA	DR7+	—	GFP	39	4	17	36	23	9
F002Rmel CIITA	DR7+	—	gp100	132	59,647	4,670	56,194	26	18
1909mel CIITA	DR7+	—	—	98	51,344	1,752	577	16	17
624mel CIITA	DR4+ DR7+	—	—	4,308	5,847	266	1,557	112	11
526mel CIITA	DR4+	—	—	5,838	19	16	24	203	41
1861mel CIITA	DR4+	—	—	4,860	13	33	46	72	40
Patient 3 PBMC	DR4+ DR7+	—	—	71	50	31	27	23	15
Patient 3 PBMC	DR4+ DR7+	NY-ESO-1	—	2,128	8,421	85	1,146	47	32
Patient 3 PBMC	DR4+ DR7+	gp100	—	15,133	69,269	3,761	20,183	1,630	10

^*IFN-γ secretion (pg/mL) in 20-hour coculture supernatants of target cells with T lymphocytes.

^†Synthetic peptides were preloaded onto EBV-B cells at 50 μmol/L ~3 hours at 37°C, and recombinant proteins were preloaded onto PBMC at 10 μg/mL overnight at 37°C.

^‡The indicated genes were transformed into 293 cells or retrovirally transduced into F002Rmel CIITA cells.

Note. Underlined values indicate that IFN-γ secretion in response to target cells expressing gp100 and either HLA-DRβ1*0701 or HLA-DRβ1*0401 was ≥50 pg/mL and at least twice background with relevant control target cells.