TABLE 5.
Functional Assay to Test for HLA-A2 Expression by Autologous Cell Lines 1928mel and 1973mel
M6* | LB7.4.2† | ||
---|---|---|---|
Targets | Pulsed with | (pg/mL IFN-γ) | |
1928mel | None | 10 | 969 |
1928mel | gp100:280–288 | 14 | 931 |
1928mel | MART-1:27–35 | 687 | 953 |
1973mel | None | 5 | 728 |
1973mel | gp100:280–288 | 10 | 723 |
1973mel | MART-1:27–35 | 6 | 507 |
T2 | None | 6 | 12 |
T2 | gp100:280–288 | 7 | 11 |
T2 | MART-1:27–35 | 923 | 11 |
Medium | 9 | 8 |
M6 is an HLA-A2-restricted, MART-1-specific CTL clone.
LB7.4.2 is a control allogeneic CD4+ clone that recognized 1928mel and 1973mel.