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. 2007 Dec 13;149(3):1091–1102. doi: 10.1210/en.2007-1498

Figure 4.

Figure 4

DNA binding of PR or AR to the proximal FSHβ promoter is required for the synergistic activation by steroids and activin. A, The −1000FSHβluc reporter plasmid was transiently cotransfected with wild-type (WT) or mutant PR (PRC577A). B, The −1000FSHβluc reporter was transiently cotransfected into LβT2 cells with the wild-type or mutant AR (ARC562G). C and D, The wild-type −1000FSHβluc reporter or the −381 mutant was transiently cotransfected into LβT2 cells with PR or AR, respectively. After overnight starvation in serum-free media, cells were treated with vehicle, 10 ng/μl activin, 100 nm steroid, or both hormones for 24 h. *, Significant differences from the vehicle-treated control; †, synergistic interaction as defined by a two-way ANOVA (P < 0.05).