Figure 5.

Activation of FoxO1 suppresses IGF-II expression. A, IGF-II in conditioned media of differentiating FoxO1–3A-ER cells was analyzed by ELISA, and the relative amounts of IGF-II are shown. The average results from three independent experiments are shown, and the error bars represent sd. B, IGF-II ME luciferase reporter was transfected into FoxO1–3A-ER cells, and the luciferase activity was measured at various time points of differentiation in the presence or absence of 4-HT. Data shown are from a representative experiment with triplicate cell treatment for each condition; four independent experiments were performed with similar results.