Table 2.
Bone histomorphometric measurements in Ro1-expressing mice and littermate controls
Mice | Ob.S/BS (%) | Oc.S/BS (%) | MS/BS (%) | MAR (mcm/d) | BFR (mcm/d) |
---|---|---|---|---|---|
Littermate controls, male | 4.60 ± 0.96 | 0.22 ± 0.04 | 73.77 ± 3.43 | 0.5 ± 0.02 | 36.46 ± 1.44 |
Ro1 × Col I-2.3-tTA, male | 4.49 ± 0.71 | 0.18 ± 0.04 | 67.08 ± 6.28 | 0.35 ± 0.04a | 23.98 ± 3.83a |
Littermate controls, female | 18.68 ± 2.15 | 0.68 ± 0.10 | 82.65 ± 2.41 | 0.53 ± 0.04 | 44.32 ± 4.57 |
Ro1 × Col I-2.3-tTA, female | 15.37 ± 1.54 | 0.89 ± 0.18 | 75.27 ± 2.47a | 0.426 ± 0.04a | 31.88 ± 2.47a |
Ro1 expression was induced by doxycycline withdrawal at 4 wk of age. Analyses of osteoblast surface (Ob.S/BS) and osteoclast surface (Oc.S/BS) were performed in the distal femur of 12-wk-old mice, male (n = 13) and female (n = 14). Measurements of percent mineralizing surface (MS/BS), mineral apposition rate (MAR), and bone formation rate (BFR) were performed on tibias of 8-wk-old mice, male (n = 4) and female (n = 8). Mice were withdrawn from doxycycline at 4 wk of age.
Significant increase or decrease in the parameter relative to the value in the corresponding littermate control group (P < 0.05).