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. 2007 Nov 26;149(3):1323–1328. doi: 10.1210/en.2007-1321

Figure 2.

Figure 2

5-HT2CRs are coexpressed with POMC in the ARC. A, Wild-type (+/+) mice exhibit endogenous POMC mRNA using ISHH with 35S-POMC (identified by clusters of black grains) but no β-galactosidase activity (X-gal stain of blue cytoplasm) in the ARC. B, Pomc tau-lacZ+/− mice display 35S-POMC in every neuron positive for X-gal. C, Higher level magnification of wild-type+/+ mouse ARC, showing 35S-POMC grains but no X-gal stain. D, Higher-level magnification of Pomc tau-lacZ+/− mouse ARC, showing a typical cell containing both 35S-POMC grains and X-gal stain (arrow). E–F, Fluorescent IHC for 5-HT2CR (E) and β-galactosidase (F) in Pomc tau-lacZ+/− mouse ARC. G, Merge of E and F, showing cells coexpressing 5-HT2CR and β-galactosidase (arrows). Scale bar (B), 200 μm and applies to A; (D), 20 μm and applies to C; (G), 20 μm and applies to E–G.