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. 2008 Mar 17;205(3):611–624. doi: 10.1084/jem.20070544

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Copyright © 2008, The Rockefeller University Press

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Figure 2. — Loss of FOG-1 results in an increased number of YSMC progenitors in vivo. (A) Yolk sac cultures from WT, FOG-1^−/−, GATA-1^V205G(KI) male, GATA-2^{V296G/V296G(KI/KI)}, and compound GATA-1^KI, GATA-2^KI/KI male embryonic day 9.5 embryos. Shown are numbers of mast cell, mixed (Mix), megakaryocytic (Meg), erythroid, and GM colonies per 10⁵ cells plated and cultured in 2 IU/ml EPO, 5 ng/ml TPO, and rat 50 ng/ml SCF for 7 d. The number of animals analyzed for each is indicated above the graphs. Error bars represent the SEM. (B) Colony and cell morphology of mast cell colonies from GATA-1^KI, GATA-2^KI/KI male embryos. (a) Brightfield appearance of colony. Bar, 50 μm. (b) May-Grunwald-Giemsa stain of cytospun cells. Bar, 10 μm. (c) Toluidine blue stain of cytospun cells. Bar, 10 μm.