Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Mar 17;205(3):657–667. doi: 10.1084/jem.20071734

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2008, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — DC migration from infected sites is inhibited by host-derived SPARC. (A) Endogenous DC migration is restored in SPARC^−/− mice. 10⁷ fluorescent latex (LX) microspheres/site were injected i.d. in mice in four different sites in the presence or absence of 10⁷ CFU of S. typhimurium. Mice were killed at day 3 after treatment and the total number of IA/IE⁺ cells carrying FITC-latex particles per DLNs was assessed after acquisition of the whole sample. The efficiency of latex⁺ cell migration (the number of latex⁺ cells in DLN of mice injected only with latex particles is considered as 100% of migration) to the DLNs is shown. The difference between efficiency of migration of latex⁺ cells in the presence of bacteria (LX + BT) in SPARC^+/+ (white bars) versus SPARC^−/− (black bars) mice is highly significant (***, P < 0.0001). This is one experiment representative of three each with three mice per group. (B) BM-DCs from SPARC^+/+ and SPARC^−/− display the same kinetic of activation in vitro. BM-DCs were generated in GM-CSF–conditioned medium and activated with bacteria at a multiplicity of infection of 10:1 or with 1 μg of LPS. Up-regulation of costimulatory molecules was evaluated by flow cytometry at the indicated time points. One of two similar experiments is shown. (C) DC migration is inhibited by host-derived SPARC. BM-DCs from SPARC^+/+ and SPARC^−/− were loaded with green and red latex beads (LX), respectively. Mice were injected i.d. with BM-DCs from both strains in the presence or absence of bacteria (BT), and cell migration to the DLNs was assessed 2 d after injection. (left) The mean total efficiency of migration ± the SD of six different mice/group is shown. The difference in DC migration efficiency in the recipient SPARC^+/+ mice in the presence of bacteria is not statistically significant (P > 0.05). (right) Representative dot plots showing total migrated cells from single DLNs in the different conditions. Recipient line shows the recipient background where BM-DCs from the two donor strains were injected in the presence or absence of bacteria (BT). (top) Dot plots show the recovery of Red LX laden SPARC^−/− BM-DCs; (bottom) dot plots show the recovery of Green LX laden SPARC^+/+ BM-DCs.