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. 2008 Mar 17;205(3):657–667. doi: 10.1084/jem.20071734

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Copyright © 2008, The Rockefeller University Press

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Figure 5. — Restored DC migration correlates with enhanced bacterial counts into DLN and increased T cell proliferation. (A) Bacterial load in SPARC^−/− is higher than that in SPARC^+/+ DLNs at days 2 and 3 after injection. DLNs of mice treated with bacteria were lysed with Na deoxycholate and plated on LB agar. Bacterial CFU were counted after overnight incubation at 37°C. The difference between bacterial counts at 48 and 72 h in DLN from SPARC^+/+ versus SPARC^−/− mice is highly significant (*, P < 0.01; **, P < 0.001). One of two similar experiments is shown. (B) OVA-expressing recombinant bacteria induce proliferation of OVA-specific CD4 T cells in SPARC^−/−, but not in SPARC^+/+ mice. SPARC WT and KO recipient mice were adoptively transferred with 3 × 10⁶ CFSE labeled DO11.10 CD4⁺ T cells, and were injected i.d. 24 h later with recombinant S. typhimurium–expressing (SL-OVA) or not expressing (SL-pGEX) OVA. Proliferation of transferred T cells in DLNs was assessed 3 d after S. typhimurium injection. The number of CFSE⁺ proliferating cells is shown ± the SD of 8 mice per group. The difference in T cell proliferation in SPARC^+/+ versus SPARC^−/− mice is highly significant (***, P < 0.001).