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. 2008 Mar 17;205(3):657–667. doi: 10.1084/jem.20071734

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Copyright © 2008, The Rockefeller University Press

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Figure 7. — SPARC^−/− mice display reduced systemic bacterial dissemination. (A) Bacterial dissemination was evaluated in SPARC^+/+ and SPARC^−/− animals injected intradermally with 10³ CFU of pathogenic S. typhimurium. DLNs, nonDLNs, and spleens were lysed and plated at different time points. Total bacterial count/organ is shown ± the SD of 12 different mice per group. The difference in bacterial recovery in SPARC^+/+ and SPARC^−/− animals is statistically significant (*, P < 0.01). (B) Intradermal immunization with attenuated S. typhimurium can protect SPARC^−/− but not SPARC^+/+ mice from subsequent challenge with pathogenic S. typhimurium. SPARC^+/+ and SPARC^−/− mice were vaccinated subcutaneously with 2 × 10³ CFU of attenuated S. typhimurium or with PBS as a control. 2 wk after vaccination, all mice were challenged i.v. with 10³ CFU of pathogenic S. typhimurium. Only SPARC-deficient mice vaccinated with bacteria survive upon challenge with virulent S. typhimurium. Kaplan-Meyer survival curves are shown. This is one representative of four different experiments each performed on five mice per group. The difference between the SPARC^−/−-vaccinated group versus all the others is statistically significant (P < 0.01, Log-Rank).