Figure 8.

Expression of Zeb2 NAT and 5′-UTR correlates closely in cell lines and human tumors. RNA was obtained from the indicated cell lines (A), treated with TGF-β (5 ng/mL) when appropriate, or from a panel of 14 colon adenocarcinomas (B). The expression of the different transcripts was performed by semiquantitative RT–PCR (A) or qRT–PCR (B) as indicated in the Materials and Methods. B, top, shows the Spearman coefficient and the P index corresponding to the association of expression of the different transcripts; the bottom shows the results corresponding to the most significant correlations, indicating the Pearson correlation coefficient.