Table 1.
Sequence and characteristics of the oligonucleotides used for RT–PCR analysis
The oligonucleotides correspond to the sequences referred to as NM014795.2 (Zeb2, with the indicated intron as explained in the Materials and Methods), AY015988 (Renilla Luciferase), AB261988 (Firefly Luciferase), NM 005985.2 (Snail1), NM 004360.2 (E-cadherin/CDH1), NM 016269.2 (LEF1), NM 212482 (Fibronectin), NM 000194.1 (HPRT), NM004168.1 (SDHA), NM 009864 (murine Cdh1), and NM011427 (murine Snail1). Unless indicated, sequences correspond to the human genes. The use of these oligonucleotides for semiquantitative (sq) or quantitative (q) RT–PCR is indicated.