Rec8 localization in wild type. Rec8-GFP staining (left) and corresponding DAPI (right) from karyogamy (A; the two fusing haploid nuclei show short segments of Rec8) through early leptotene (B), late leptotene with aligned homologs (C), pachytene (D), diffuse stage (E), diplotene (F), and prophase of the second division (G). (nu) Nucleus. Bar, 5 μm.