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. 2008 Mar 15;22(6):796–809. doi: 10.1101/gad.459308

Figure 2.

Figure 2.

Prophase phenotypes of wild type and rec8Δ. The two top rows show wild-type (A,C) and rec8Δ (B,D) leptotene nuclei stained by Spo76-GFP (A,B), and by MPM-2 and corresponding DAPI (C,D). (E–H) Rad51-GFP foci. They are numerous in early leptotene nuclei of wild type (E) and rec8Δ (G and corresponding DAPI). (F,H) In both strains, foci (arrows) are located between aligned homolog axes stained here by Spo76-GFP. (I–K) Bouquet formation in wild type (I) and rec8Δ (J,K). Note that wild-type chromosome ends are all clustered in a small area of the nuclear envelope (I), while mutant telomeres are only partially clustered (J,K). Wild-type metaphase I exhibits seven bivalents (L), while rec8Δ early anaphase I (M) shows scattered 28 chromatids and a smaller DAPI dot (arrow), corresponding to a broken chromatid. Bars, 5 μm.