Table 1.
Effect of chlorpromazine and pentoxifylline on plasma extravasation and release of TNF-α induced by β-toxin in the dorsal skin of mice
| Drugs | Toxin | Plasma extravasation (μl per site) | Production of TNF-α (ng g−1 of tissue) |
|---|---|---|---|
| Vehicle | − | 4.1±1.2 | 48.1±5.1 |
| Vehicle | + | 32.3±5.6 | 232.5±10.1 |
| Chlorpromazine (5 mg kg−1) | − | 5.2±2.2 | 41.3±4.9 |
| Chlorpromazine (1 mg kg−1) | + | 19.5±6.3* | 115.4±10.9* |
| Chlorpromazine (5 mg kg−1) | + | 9.7±4.8** | 76.7±6.3** |
| Pentoxifylline (5 mg kg−1) | − | 5.8±3.1 | 38.8 ±3.9 |
| Pentoxifylline (1 mg kg−1) | + | 15.9±7.5* | 96.1±9.4* |
| Pentoxifylline (5 mg kg−1) | + | 8.2±2.8** | 76.2±6.3** |
Abbreviations: i.d., intradermally; TNF-α, tumour necrosis factor-α.
Mice were injected intraperitoneally with chlorpromazine (1 and 5 mg kg−1), pentoxifylline (1 and 5 mg kg−1) or vehicle 60 min before the injection of the toxin. β-Toxin (50 ng per site) or saline (0.05 ml per site) was injected i.d. Plasma extravasation and TNF-α were evaluated as described in Methods. Values are mean±s.e.mean, n=6.
*P<0.05, compared with vehicle plus toxin.
**P<0.01, compared with vehicle plus toxin.